Cytoskeleton's Signal-Seeker PTM detection tools Promo

15% discount on listed items

Validity: 15th May 2022 to 30th Jun 2022

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Signal-Seeker Detection Tools > Acetyl-Lysine Tools 

Post-translational acetylation occurs on the epsilon amino group of lysine residues as a reversible and highly dynamic PTM that is known to be a key regulator in multiple cellular events, including chromatin structure, transcription, metabolism, signal transduction and cytoskeletal regulation.  

Available Products:

AAC01   AAC01-S   AAC02   AAC02-S   AAC03   AAC03-S   AAC03-HRP   AAC03-HRP-S  

AAC04-beads   BK163   BK163-S   CIG02-beads   AACX1

Image by Louis Reed

Signal-Seeker Detection Tools > Phosphotyrosine Tools

Tyrosine phosphorylation, a reversible process, is a frequent post-translational modification of proteins and is crucial in mediating signal transduction in eukaryotic cells after exposure to cytokines and growth factors.  Signal-Seeker™ phosphotyrosine products provide a comprehensive system to validate and study novel phosphotyrosine modifications for any target protein.

 

Available Products:

APY03   APY03-S   APY03-HRP   APY03-HRP-S   APY03-Beads   BK160   BK160-S

CIG01-beads

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Signal-Seeker Detection Tools > SUMOylation 1 Tools

SUMOylation is a dynamic and reversible process that plays pivotal roles in transcriptional regulation, chromatin remodeling, response to stress and regulation of mitosis. Signal-Seeker™ SUMOylation 1 products provide a simple and effective way to determine if your target protein is modified and regulated by this PTM. 

 

  • Quickly detect SUMO 1 modifications of novel target proteins with our comprehensive SUMOylation 1 Detection Kit:  No mass Spectrometry necessary

Available Products:

ASM01   ASM01-S   ASM11-Beads   BK165   BK165-S   CIG03-beads 

Image by Testalize.me

Signal-Seeker Detection Tools > SUMOylation 2/3 Tools

SUMOylation is a dynamic and reversible process that plays pivotal roles in transcriptional regulation, chromatin remodeling, response to stress and regulation of mitosis. Signal-Seeker™ SUMOylation 2/3 products provide a simple and effective way to determine if your target protein is modified and regulated by this PTM. 

Available Products:

ASM23   ASM23-S   ASM23-HRP   ASM23-HRP-S   ASM24   ASM24-S   ASM24-Beads   

BK162   BK162-S   

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Signal-Seeker Detection Tools > SUMOylation 2/3 Tools

Protein ubiquitination is a regulatory mechanism that controls protein:protein interactions, protein degradation, and spatial localization, which is mediated by chain specific poly-ubiquitination and/or mono-ubiquitination. Signal-Seeker™ ubiquitination tools have the unique ability to capture both mono- and poly-ubiquitination for any target protein.

 

 

 

  • Pan ubiquitin antibody detects a broad range of ubiquitinated proteins including K48, K63, and M1 poly-ubiquitin chains

Available Products:

AUB01   AUB01-S   AUB01-XL   AUB01-HRP   AUB01-HRP-S   BK161   BK161-S   

CUB02-beads   UBA01-beads 

Actin Products > Protein

Offering unmatched activity and purity in the most comprehensive actin portfolio. 

  • Choice of >95% or >99% pure

  • Labeled or unlabeled proteins

  • Cytoplasmic, and cardiac, skeletal and smooth muscle sources

  • Bulk quantities available

Available Products:

MB201   MB201-XL   MIC01   MIC01-XL   MXA95   MXA95-XL   MPAX1   MPAX1-XL

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Signal-Seeker Detection Tools > BlastR™ Filter System

The BlastR™ lysis filtration system allows users to capture proteins from all cellular compartments using a denaturing buffer to obtain a more complete protein profile relative to standard non-denaturing buffers.  One significant drawback with denaturing buffers is the viscous genomic DNA contamination that can interfere with protein analysis, migration of proteins on an SDS-PAGE gel, and interference with immunoprecipitation assays.

 

BlastR™ filters remove viscous genomic DNA from denaturing cell lysis buffer extracts while reducing processing time to less than one minute. The proprietary filtration technology is based on a compressible polyurethane filter with optimal pore size for capturing genomic DNA. The compressible nature of the filter allows for maximal recovery of sample which is usually 90% of the original volume.

 

Two versions are available: 1) filters plus buffers version (Cat. # BLR01, includes; BlastR™ Lysis Buffer), which was developed as an integrated component of the Signal-Seeker™ PTM detection product line. The BlastR™ lysis buffer was optimized as a "universal" immunoprecipitation buffer for low abundance PTM modified proteins. Additionally, it effectively isolates proteins from all cellular compartments making it useful for western blot applications.  2) Filters only (Cat. # BLR02, which allows the operator to use their own denaturing buffer such as guanidine, urea or high SDS based extraction buffers that usually produce copious viscosity.

Available Products:

BLR01   BLR02   BLR03